Optogenetics: Methods and Protocols (Methods in Molecular Biology)

Author: Arash Kianianmomeni
Publisher: Humana Press Inc.
Category: Anatomy, Medical Genetics, Neurology & Clinical Neurophysiology, Science: General Issues, Light (optics), Genetics (non-medical), Neurosciences, Molecular Biology
Book Format: Hardcover

This volume provides researchers with up-to-date protocols and takes a

close look at current research and promising applications. Optogenetics:

Methods and Protocols contains a collection of recently developed technical

protocols on optogenetic applications in neuroscience, brain mapping, treatment

of neurological disorders, and restoration of visual function. Several

introductory and discussion chapters offer a wide overview about sources and

diversity of optogenetic tools, design strategies, and potential applications

in other fields like plant research. Written in the highly successful Methods

in Molecular Biology series format, chapters include introductions to their

respective topics, lists of the necessary materials and reagents, step-by-step,

readily reproducible laboratory protocols, and tips on troubleshooting and

avoiding known pitfalls.



Cutting edge and thorough, Optogenetic: Methods and Protocols, delivers

an interdisciplinary view of optogenetic applications and its potential to

develop as a valuable tool for basic research, as well as biomedical and

biotechnological applications.







Table Of Contents
1.

Optogenetics:

Basic Concepts and Their Development

Yong Ku Cho and Dan Li
2.

Natural

Resources for Optogenetic Tools

Tilo Mathes
3. Algal Photobiology: A Rich Source of

Unusual Light Sensitive Proteins for Synthetic Biology and Optogenetics

Arash Kianianmomeni and Armin

Hallmann
4. Reversible Photoregulation of gene

Expression and Translation

Shinzi Ogasawara
5. Controlling Protein Activity and

Degradation Using Blue-Light

Anne P. Lutz, Christian Renicke, and

Christof Taxis
6. Photo-Control of Protein Function Using

Photoactive Yellow Protein

Jakeb M. Reis and G. Andrews Woolley
7. A Fluorometric Activity Assay for

Light-Regulated Cyclic-Nucleotide-Monophosphate Actuators

Charlotte Helene Chumacher, Heinz G.

Koerschen, Christopher Nicol, Carlos Gasser, Reinhard

Seifert, Martin Schwarzel, and Andreas Moeglich
8. Optogenetic Control of Pancreatic Islets

Thomas M. Reinbothe and Ines G.

Mollet
9. Optogenetics in Plants: Red/Far-Red Light

Control of Gene Expression

Rocio Ochoa-Fernandez, Sophia L.

Samodelov, Simon M. Brandl, Elke Wehinger, Konrad Muller, Wilfried Weber, and

Matias D. Zurbriggen
10. Perfect Channelrhodopsins: A User Guide

Jonas Wietek and Matthias Prigge
11. Optogenetics in Drosophila Neuroscience

Thomas Riemensperger, Robert J.

Kittel, and Andre Fiala
12. Optogenetic Control of Mammalian Ion

Channels with Chemical Photoswitches

Damien Lemoine, Romain Durand-de

Cuttoli, and Alexandre Mourot
13. Optogenetic Modulation of Locomotor

Activity on Free Behaving Rats

Kedi Xu, Jiacheng Zhang, Songchao

Guo, and Xiaoxiang Zheng
14. Combines Optogenetic and Chemogenetic

Control of Neurons

Ken Berglund, Jack K. Tung, Bryan

Higashikubo, Robert E. Gross, Christopher I. Moore, and Ute Hochgeschwender

15. Intracranial Injection of an Optogenetics

Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in

Rat Brain Cortex

Christopher Pawela, Edgar DeYoe, and

Ramin Pashaie
16. An Optomized Calcium-Phosphate Transfection

Method for Characterizing Genetically Encoded Tools in Primary Neurons

Shiyao Wang and Yong Ku Cho
17. Optogenetic Approaches for Mesoscopic Brain

Mapping

Michael Kyweriga and Majid H.

Mohajerani
18. Optogenetic Tools for Confined Stimulation

in Deep Brain Structures

Alexandre Castonguay, Sebastien

Thomas, Frederic Lesage, and Christian Casanova
19. Remote Patterning of Transgene Expression

Using Near Infrared-Responsive Plasmonic Hydrogels

Francisco Martin-Saavedra and Nuria

Vilaboa
20. Optogenetic Light Crafting Tools for the

Control of Cardiac Arrhythmias

Claudia Richter, Jan Christoph,

Stephan E. Lehnart, and Stefan Luther
21. Inscribing Optocal Excitability to

Non-Excitable Cardiac Cells: Viral Delivery of Optogenetic Tools in Primary

Cardiac Fibroblasts

Jinzhu Yu and Emilia Entcheva
22. Optogenetic Engineering of Atrial

Cardiomyocytes

Iolanda Feola, Alexander Teplenin,

Antoine A.F. de Vries, and Daniel A. Pijnappels
23. A Multi-Channel Recording System with

Optical Stimulation for Closed-Loop Optogenetic Experiments

Carmen Bartic, Francesco P.

Battaglia, Ling Wang, Thoa T. Nguyen, Henrique Cabral, and Zaneta Navratilova
24. Optogenetic Control of Fibroblast Growth

Factor Receptor Signaling

Nury Kim, Jin Man Kim, and Won Do Heo
25. Protein Inactivation by Optogenetic

Trapping in Living Cells

Hyerim Park, Sangkyu Lee, and Won Do

Heo
26. Optogenetic Manipulation of Selective

Neural Activity in Free-Moving Drosophila

Adults

Po-Yen Hsiao, Ming-Chin Wu, Yen-Yin

Lin, Chein-Chung Fu, and Ann-Shyn Chiang
27. Guidelines for Photoreceptor Engineering

Thea Ziegler, Charlotte Helene

Schumacher, and Andreas Moeglich

(BK-9781493935109)

SKU BK-9781493935109
Barcode # 9781493935109
Brand Humana Press Inc.
Artist / Author Arash Kianianmomeni
Shipping Weight 0.9800kg
Shipping Width 0.190m
Shipping Height 0.030m
Shipping Length 0.260m
Assembled Length 26.400m
Assembled Height 2.900m
Assembled Width 18.700m
Type Hardcover

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